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Anti-PAX6 antibody [AD2.38] (ab78545)抗轉(zhuǎn)錄因子Pax6抗體產(chǎn)品信息：

• Product nameAnti-PAX6 antibody [AD2.38]See all PAX6 primary antibodies ...
• Description

  Mouse monoclonal [AD2.38] to PAX6

• SpecificityThis antibody recognizes both products of the two major alternatively spliced forms.
• Tested applicationsIHC-Fr, Sandwich ELISA, IHC-P, Flow Cyt, ICC/IF, IHC-FoFr more details
• Species reactivity

  Reacts with: Mouse, Human
  Predicted to work with: Rat, Chicken

• Immunogen

  Recombinant full length protein Human PAX6

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• Concentration 100 µg at 1 mg/ml

• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberAD2.38
• IsotypeIgG1
• Research Areas
  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker
  • Neuroscience
  • Neurology process
  • Neural Signal Transduction
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • PAX
  • Stem Cells
  • Lineage Markers
  • Ectoderm
  • Stem Cells
  • Lineage Markers
  • Endoderm
  • Developmental Biology
  • Lineage specification
  • Endoderm
  • Developmental Biology
  • Lineage specification
  • Ectoderm
  • Developmental Biology
  • Organogenesis
  • Gut development
  • Pancreas development

Applications

Our Abpromise guarantee covers the use of ab78545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionTranscription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
• Tissue specificityFetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
• Involvement in diseaseDefects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
  Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
  Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
  Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
  Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
  Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
  Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
  Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
• Sequence similaritiesBelongs to the paired homeobox family.
  Contains 1 homeobox DNA-binding domain.
  Contains 1 paired domain.
• Developmental stageExpressed in the developing eye and brain.
• Post-translational
  modificationsUbiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
• Cellular localizationNucleus.
• Information by UniProt

• Database links

  • Entrez Gene: 5080 Human
  • Entrez Gene: 18508 Mouse
  • Entrez Gene: 25509 Rat
  • Omim: 607108 Human
  • SwissProt: P47237 Chicken
  • SwissProt: P26367 Human
  • SwissProt: P63015 Mouse
  • SwissProt: P63016 Rat

• Alternative names

  • AN 2 antibody
  • AN antibody
  • AN2 antibody

Anti-PAX6 antibody [AD2.38] images

• 

  Sandwich ELISA - Anti-PAX6 antibody [AD2.38] (ab78545)

  Standard Curve for Pax6; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [AD2.38] to PAX6 (ab78545) at 5 ug/ml and Detector Antibody Rabbit polyclonal to PAX6 (ab82510) at 0.5 ug/ml.

• 

  Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (ab78545)This image is courtesy of an anonymous Abreview

  Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.
  Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by NO. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.

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